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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Improvement of Fab expression by screening combinatorial synonymous signal sequence libraries

Fig. 5

Effect of leucine codons on expression at position Leu-5 in N–0 library. The clones in N–0 library were pooled according to the codon identity at position L5 (CTA, CTC, CTG, CTT or TTG). The average expression levels and the standard deviation of each pool are represented. According to the Kruskal–Wallis analysis, CTG codon (n = 6) at position Leu-5 caused significant decrease in expression when compared to CTA codon (n = 6) (Adj. Sig. p = 0.005) and CTT codon (n = 7) (Adj. Sig. p = 0.035) in N–0 library. Sampling sizes of CTC and TTG codons were 7 and 5, respectively. Statistically significant (p < 0.05) differences to CTG codon are indicated with asterisk (*)

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