Fig. 4

Correlation between the relative adaptiveness values and the expression levels of immunoreactive Fab fragment. Black line (with diamond markers) indicates the p-value at given position in the light chain PelB and grey line (with triangle markers) indicates the p-value at given position in the heavy chain PelB. Dashed black line (with square markers) indicates the Spearman rank correlation coefficient at given position in the light chain PelB and dashed grey line (with sphere markers) indicates the Spearman rank correlation coefficient at given position in the heavy chain PelB. Horizontal dashed black and grey lines indicate the levels below which the correlations are significant after Bonferroni correction for multiple correlations in N–0 library (0.00714) and in H–0 library (0.00625), respectively. Positions where solid black or grey line goes below the dashed vertical lines are considered as statistically significant. Spearman rank correlation coefficients (r), p-values and sampling sizes (n) of each correlation analysis at each position are shown in numerical form below the graph. Letters N, H and C denote the N–0, H–0 and C–0 library regions, respectively