13C metabolic flux analysis on roles of malate transporter in lipid accumulation of Mucor circinelloides

Table 5 Yield and consumption of NADPH in recombinant M. circinelloides strains under different nitrogen concentrations

Strains	Condition	NADPH yield				NADPH consumption
		PP pathway		POM cycle	TCA cycle	Biosynthesis
		G6PD	6PGD	ME	ICDH	Lipid	Protein	Nucleic acid
MU522	HN	0.315	0.308	0.105	0.987	0.127	1.514	0.075
MU522	LN	0.397	0.389	0.114	0.789	0.146	1.486	0.056
MU241	HN	0.349	0.332	0.084	0.941	0.141	1.482	0.082
MU241	LN	0.401	0.388	0.107	0.732	0.213	1.361	0.056
Mc-MT-1	HN	0.366	0.352	0.097	0.857	0.135	1.473	0.066
Mc-MT-1	LN	0.423	0.417	0.115	0.662	0.238	1.321	0.059
Mc-MT-2	HN	0.384	0.371	0.089	0.869	0.144	1.489	0.084
Mc-MT-2	LN	0.459	0.424	0.102	0.641	0.242	1.331	0.052

Data were obtained from the flux distribution presented in Fig. 5. Values are expressed in mol NADPH/mol glucose consumed
G6PD glucose 6-phosphate dehydrogenase, 6PGD 6-phosphogluconate dehydrogenase, ME malic enzyme, ICDH isocitrate dehydrogenase

ISSN: 1475-2859