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Fig. 8 | Microbial Cell Factories

Fig. 8

From: Engineering Pseudomonas putida for isoprenoid production by manipulating endogenous and shunt pathways supplying precursors

Fig. 8

Growth and lycopene content of P. putida KTLYC cells overproducing nDXS. KTLYC cells transformed with the indicated vectors were cultured in LB. Expression of the cloned genes was induced 1 h after inoculation by adding the indicated amounts of IPTG (mM) to the culture. After 24 h, growth was measured as optical density at 600 nm (OD600) and lycopene accumulation was calculated by measuring the absorbance at 472 nm of acetone extracts. a Cell density kinetics of KTLYC cells transformed with either p424-nDXS or empty pSEVA-424 plasmids estimated from OD600 values. b Cell density and lycopene content of the strains shown in a at 24 h. Values shown are the mean ± SD of three independent assays. Asterisks indicate statistically significant differences (T-test, P-value < 0.05) relative to KTLYC(p424) controls grown without IPTG

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