Fig. 6

Test of the P. putida PkatA promoter. a Transcript levels of ccoN, cioA and katA in KTLYC(p424-IDI-DXS) cells grown in the presence of the indicated concentrations of IPTG. The graph represents the ratio of the mRNA levels in KTLYC(p424-IDI-DXS) cells relative to KTLYC(p424) controls grown under the same conditions. Error bars indicate SD. b Lycopene accumulation in the indicated strains grown for 24 h in LB without IPTG. Values shown are the mean ± SD of three independent assays. Asterisks indicate statistically significant differences (T-test, P-value < 0.05 * or 0.01 **) relative to KTLYC(p424) controls. c Lycopene accumulation in the indicated strains grown in LB without IPTG for the indicated times (in hours). Values are shown relative to 24 h samples and represent the mean ± SD of three replicates in two independent assays