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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Engineering Pseudomonas putida for isoprenoid production by manipulating endogenous and shunt pathways supplying precursors

Fig. 4

Cell density and lycopene content of P. putida KTLYC cells overproducing DXS. Overnight cultures of KTLYC cells transformed with the indicated vectors were inoculated in LB. Expression of the cloned genes was induced 1 h after inoculation by adding the indicated amounts of IPTG (mM) to the culture. After 24 h, growth was estimated from optical density at 600 nm (OD600) and lycopene accumulation was calculated by measuring the absorbance at 472 nm of acetone extracts. a Cell density kinetics of KTLYC cells transformed with either p424-DXS or empty pSEVA424 plasmids, estimated from OD600 values. b Cell density and lycopene content of the strains shown in a at 24 h. c Cell density kinetics of KTLYC cells transformed with either p424-IDI-DXS or empty pSEVA424 plasmids, estimated from OD600 values. d Cell density and lycopene content of the strains shown in c at 24 h. Values shown are the mean ± SD of three independent assays. Asterisks indicate statistically significant differences (T-test, P-value < 0.05 * or 0.01 **) relative to KTLYC(p424) controls grown without IPTG

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