Fig. 10

Cell growth and lycopene accumulation in P. putida KTLYC cells overproducing DXS and DXR. Overnight cultures of KTLYC cells transformed with the indicated vectors were inoculated in LB and induced to express the cloned genes with 0.1 mM IPTG added 1 h after inoculation. a Cell density kinetics of the indicated strains estimated from OD₆₀₀ values. b Cell density and lycopene content of the strains shown in a at 24 h. Values shown are the mean ± SD of three independent assays. Asterisks indicate statistically significant differences (T-test, P-value < 0.05 * or 0.01 **) relative to KTLYC(p424) controls