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Table 2 Relative extracellular amylase activity in growth medium of Bacillus subtilis 168 \(\Delta\)sigF co-expressing heterologous amylases and heterologous prsA genes

From: Identification and optimization of PrsA in Bacillus subtilis for improved yield of amylase

Origin of heterologous prsA gene inserted into the pel locus

Origin of heterologous amyE homologue inserted into the amyE locus

None (only wild type prsA gene)

+ B. subtilis prsA

+ B. licheniformis \(prsA^{\mathrm{Bl}}\)

+ B. amyloliquefaciens \(prsA^{Ba}\)

+ G. stearothermophilus \(prsA^{Gs}\)

+ B. sonorensis L12 \(prsA^{Bson}\)

+ B. NSP9.1 \(prsA^{BN}\)

Bacillus subtilis (amyE)

1 (0.09)

1.25 ( 0.11)

0.91 (0.06)

1.18 (0.13)

1.17 (0.18)

0.84 (0.07)

0.56 (0.16)

Bacillus licheniformis (amyL)

1 (0.08)

1.20 (0.15)

1.40 (0.08)

1.48 (0.06)

1.44 (0.08)

1.46 (0.10)

1.06 (0.17)

Bacillus amyloliquefaciens (amyQ)

1 (0.08)

1.18 (0.13)*

1.27 (0.18)

1.19 (0.05)*

0.29 (0.01)

0.94 (0.007)

0.89 (0.02)

Geobacillus stearothermophilus (amyS)

1 (0.13)

1.40 (0.22)

2.19 (0.15)

2.41 (0.21)

1.01 (0.05)

0.93 (0.03)

1.74 (0.03)

Bacillus sonorensis L12

1 (0.1)

0.95 (0.28)

2.35 (0.41)

2.07 (0.25)

1.72 (0.18)

2.54 (0.19)

1.94 (0.44)

Bacillus NSP9.1

1 (0.17)

1.12 (0.26)

1.77 (0.15)

1.64 (0.16)

1.38 (0.1)

1.26 (0.13)

1.50 (0.10)

  1. Values are calculated as the mean of six determinations, normalized to the level of amylase activity in the strain expressing each amylase with no added prsA gene. Results highlighted in italics are statistically significant (\(p<0.05\)) for a pairwise t-test Bonferroni corrected regarding the strain overexpressing the amylase with no added prsA expression. Results marked with an asterisk have a p value \(0.10<p>0.05\)