Engineering protein production by rationally choosing a carbon and nitrogen source using E. coli BL21 acetate metabolism knockout strains

Table 3 Specific acetate production and consumption rates

	Acetate production rate (mmol/gh)				Acetate consumption rate (mmol/gh)
	Glucose TB7^a	Glycerol TB7^a	Glucose MM9^a	Glycerol MM9^a	Glucose TB7^a	Glycerol TB7^a	Glucose MM9^a	Glycerol MM9^a
wt	13.62	7.80	0.35	0.14	− 7.02	− 5.16	−0.11	−0.11
ΔpatZ	9.79	13.90	0.27	0.16	− 6.85	− 6.80	−0.13	−0.07
ΔcobB	16.49	11.10	0.54	0.38	− 1.70	− 2.04	−0.20	−0.08
Δacs	11.06	10.54	0.38	0.18	− 1.70	− 2.29	−0.20	−0.02
ΔackA	0.12	2.79	0.09	0.11	−0.21	− 1.87	−0.11	−0.05
Δpta	0.17	3.64	0.08	0.07	−0.20	− 1.41	−0.06	−0.06

^aEach value is the average of three independent experiments with a standard deviation of less than 10%

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