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Box 2. | Microbial Cell Factories

Box 2.

From: Metabolic fluxes-oriented control of bioreactors: a novel approach to tune micro-aeration and substrate feeding in fermentations

Box 2.

Flux-based micro-aerated control (FMC): equations for updating fresh medium feeding and air flow rates. The values for constants and other conditions are: \({\text{y}}_{{{\text{CO}}_{ 2} , {\text{ N}}_{ 2} }}\) = 0.01*10−2; Tin = 294.25 K; Pout = 1 atm; R = 0.08206*10−3 L atm mmol−1 K−1. Qgas,out, \({\text{y}}_{{{\text{CO}}_{ 2} , {\text{ in}}}}\), and \({\text{y}}_{{{\text{O}}_{ 2} , {\text{ in}}}}\) were estimated through mass balance. The variables \({\text{y}}_{{{\text{CO}}_{ 2} , {\text{ out}}}}\), \({\text{y}}_{{{\text{O}}_{ 2} , {\text{ out}}}}\) and Tout were measured on-line and accessed through the supervisory software. \({\text{J}}_{\text{s}}^{\text{MC}}\), \({\text{J}}_{{{\text{O}}_2}}^{\text{MC}}\), \({\text{J}}_{{{\text{O}}_{2} }}^{\text{CA}}\), \({\text{J}}_{{{\text{CO}}_{2} }}^{\text{CA}}\) are expressed in mmol g DW −1  h−1. QN2, QAIR and F were manipulated and are expressed in L h−1. *Mathematical correlations in Results Metabolic Models Simulations and Correlations (Eqs. 1 and 2, respectively)

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