Table 2 Strains and plasmids used in this study
Strain or plasmid | Relevant marker | Source or references |
|---|---|---|
Escherichia coli | ||
DH5α | F−, ϕ80lacZ∆M15, ∆(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17(r −K m +K ), phoA, supE44, λ−, thi-1, gyrA96, relA1 | Lab stock |
BL21(DE3) | E. coli B, F−, dcm, ompT, hsdS(r −B m −B ), gal, λ(DE3) | Novagen |
S17-1 | thi pro hsdR recA chromosomal RP4; Tra+; Tmpr Str/Spcr | [43] |
Ralstonia eutropha | ||
H16 | Wild type | DSM 428 |
NSDG | H16 derivative; ΔphaC::phaCNSDG | [33] |
NSDG-GG | NSDG derivative; ΔnagR, nagE(G793C), PA2858-glpFKEc-h16_A2858 | This study |
NSDG-GGΔB1 | NSDG-GG derivative; ΔphaB1 | This study |
NSDG-GG-BNV | NSDG-GG∆B1 derivative; phaB1NV | This study |
NSDG-GG-BNVYF | NSDG-GG∆B1 derivative; phaB1NVYF | This study |
NSDG-GG-B2 | NSDG-GG∆B1 derivative; ∆phaB1::phaB2 | This study |
NSDG-GG-HC | NSDG-GG∆B1 derivative; ∆phaB1::had-crt2 | This study |
Plasmids | ||
pEE32 | pUC18 derivative; phaPCJAc | [10] |
pBPP | pBBR ori (broad host range), mob, Kanr, PphaP1 | [44] |
pBPP-ccrMeJ4a-emd | pBPP derivative; ccrMe, phaJ4a, emdMm | [23] |
pBPP-ccrMeJAc-emd | pBPP derivative; ccrMe, phaJAc, emdMm | This study |
pColdII | ColE1 ori, Ampr, PcspA | Takara Bio |
pColdII-phaB1 | pColdII derivative, phaB1 | This study |
pColdII-phaB2 | pColdII derivative, phaB2 | This study |
pET15b-phaB3 | pET15b derivative, phaB3 | This study |
pColdII-phaB1NV | pColdII derivative, phaB1NV | This study |
pColdII-phaB1NVYF | pColdII derivative, phaB1NVYF | This study |
pK18mobsacB-AR | pK18mobsacB derivative; phaB1 del | [33] |
pK18mobsacB-ABNVYFR | pK18mobsacB-AR derivative; phaB1NVYF ins | This study |
pK18mobsacB-ABNVR | pK18mobsacB-AR derivative; phaB1NV ins | This study |
pK18mobsacB-AB2R | pK18mobsacB-AR derivative; phaB2 ins | This study |
pK18mobsacB-AHCR | pK18mobsacB-AR derivative; had-crt2 ins | This study |