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Table 1 Kinetic parameters of PhaB paralogs and PhaB1 mutants from R. eutropha toward acetoacetyl-CoA

From: Modification of acetoacetyl-CoA reduction step in Ralstonia eutropha for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from structurally unrelated compounds

Enzyme Mutation(s) Km [μM] Vmax [U mg−1] kcat [s−1] kcat/Km [μM−1 s−1]
PhaB1   1.99 ± 0.23 162 ± 6 71.2 ± 2.7 35.8 ± 4.4
PhaB1NV N142V 58.5 ± 18.1 175 ± 31 76.7 ± 2.7 1.31 ± 0.47
PhaB1YF Y185F 86.2 ± 20.6 127 ± 17 55.8 ± 7.6 0.65 ± 0.18
PhaB1NVYF N142V/Y185F 109 ± 38 53.9 ± 8.3 23.7 ± 3.7 0.22 ± 0.08
PhaB2   2.48 ± 0.63 10.5 ± 0.6 4.90 ± 0.28 2.00 ± 0.52
PhaB3   1.27 ± 0.53 88.9 ± 8.7 40.8 ± 4.0 32.7 ± 14.0