Fig. 5

The colonization of NJAU4742 strain on cucumber roots after being treated by various FRDP. a The morphological characteristics of TgSWO knockout mutants; KO1 and KO2 were two relatively independent mutants. b The morphological characteristics of TgSWO overexpressed mutants; OE1 and OE2 were two relatively independent mutants. c The colonization of the KO mutants (KO1 and KO2) on the roots of the cucumber seedlings, with the wild-type used as a control, and each bar represent the mean of three biological replicates with SE. d The colonization of the OE mutants (OE1 and OE2) on the roots of the cucumber seedlings, with wild-type used as the control, and each bar representing the mean of three biological replicates with SE. e The colonization of NJAU4742 strain on the cucumber roots after being treated by TgSWO at different concentrations (5, 10, 15, and 20 μM), and CK was without TgSWO. f Comparison of the colonization of different strains on the cucumber roots; KO + TgSWO indicates TgSWO was added exogenously to the KO1 mutant 48 h after being inoculated. g Comparison of the colonization of the different strains on the cucumber roots, and WT + TgSWO indicates TgSWO was added exogenously to the wild-type 48 h after inoculation. h SEM observations of root colonization of NJAU4742 strain. i CLSM analysis results of GFP-marked NJAU4742 strain colonizing the cucumber roots in TgSWO (15 µM), ΔCBD (7 µM), ΔYoaJ (10 µM), and CK was without any proteins. j The quantification of root colonization of the wild-type under the different treatments. Each bar represents the mean of three biological replicates with SE. Statistically significant difference as evaluated by one-way ANOVA: *P < 0.05; **P < 0.01, ***P < 0.001