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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Surface display of hirame novirhabdovirus (HIRRV) G protein in Lactococcus lactis and its immune protection in flounder (Paralichthys olivaceus)

Fig. 1

Expression of G protein on L. lactis NZ9000. a, b Plasmid maps of constructed expression vector (by SnapGene software), arrows indicate the length and direction of the ORFs. a pSLC vector, an expression cassette with MCS; b pSLC-G vector, expressing HIRRV-G gene. c SDS-PAGE and western blotting analysis of the induced recombinant L. lactis. M: molecular mass marker; Lane 1–3: the whole cells lysate (cell) of non-induced L. lactis, induced Ll:pSLC, induced Ll:pSLC-G, respectively; Lane 4–6: the corresponding culture supernatants (sup); Lane 7–9: the corresponding western blotting of non-induced L. lactis, induced Ll:pSLC and induced Ll:pSLC-G incubated with mouse anti-rG Pab, respectively; Line 10: western blotting of induced Ll:pSLC-G incubated with mouse negative serum

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