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Table 1 Variable gRNA targets used in this study

From: CRISPR–Cas9-mediated genomic multiloci integration in Pichia pastoris

Name

Location

Sequence (5′ → 3′)

PTEF1UP-g1

Chromosome 1

GCAAGATGGTTAAAAGGTGA

PTEF1UP-g2

GAATGGGCAAGATGGTTAAA

PGAPUP-g1

Chromosome 2

ATCGATAATAGTCGCATGTG

PGAPUP-g2

TTTTAAGATTTCAATCTTGA

PFLD1UP-g1

Chromosome 3

GCGGCAGTAATTGATATCGT

PFLD1UP-g2

AGTAATTGATATCGTAGGGT

PAOX1UP-g1

Chromosome 4

AATCCAAATGTCATCATTGT

PAOX1UP-g2

GCGCCTACAATGATGACATT

PAOX1UP-g3

TGGATTTGGTTGACTCATGT

AOXTTDOWN

TGACGCTTATTATACCCTTT