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Table 2 Kinetic and stoichiometric parameters of the strain E. coli MS04 calculated at both stages in TSCC, and as a global TSCC (G) under micro-aerated conditions in mineral medium supplemented with xylose (42.5 g/L), glucose (7.5 g/L) and sodium acetate (2 g/L)

From: Xylose–glucose co-fermentation to ethanol by Escherichia coli strain MS04 using single- and two-stage continuous cultures under micro-aerated conditions

μ1 (h−1) μ2 (h−1) μG (h−1) QP1 (gEtOH/L h) YPS1 (%) QP2 (gEtOH/L h) YPS2 (%) QPG (gEtOH/L h) YPSG (%)
0.10 0.018 0.050 1.48 ± 0.04 81 ± 1.0 0.58 ± 0.04 78 ± 2.5 1.0 ± 0.01 81 ± 0.5
0.15 0.043 0.075 1.51 ± 0.04 82 ± 0.8 1.63 ± 0.07 82 ± 1.5 1.6 ± 0.03 82 ± 0.9
0.20 0.095 0.100 1.00 ± 0.04 77 ± 3.0 1.46 ± 0.09 81 ± 3.0 1.2 ± 0.05 79 ± 3.0
0.30 0.200 0.150 0.53 ± 0.06 80 ± 3.5 1.44 ± 0.07 80 ± 3.5 1.0 ± 0.05 80 ± 3.0
  1. Average values and standard errors are shown from triplicates
  2. μ1, μ2, μG: specific growth rates at stage 1 and 2 of the two-stage continuous culture, and at the continuous global culture, respectively
  3. QP1, QP 2, QPG: ethanol volumetric productivity at stage 1 and 2 of the two-stage continuous culture, and at the continuous global culture, respectively
  4. YPS1, YPS2, YPSG: ethanol overall yield at stage 1 and 2 of the two-stage continuous culture, and at the continuous global culture, respectively
  5. G: continuous global culture was considered as a single-stage continuous culture with the sum of volumes of both stages in the two-stage continuous culture and fed at the same flow rate. DG = D1/2 = D2/2