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Table 1 Kinetic and stoichiometric parameters of ethanologenic E. coli MS04 in batch and SSCC under micro-aerated conditions in mineral medium supplemented with xylose (42.5 g/L), glucose (7.5 g/L), and sodium acetate (2 g/L)

From: Xylose–glucose co-fermentation to ethanol by Escherichia coli strain MS04 using single- and two-stage continuous cultures under micro-aerated conditions

Culture D (h−1) μ (h−1) Ethanol (gEtOH/L) YPS (%, w/w) QP (gEtOH/L h) qP (gEtOH/gDCW h) YXS (gDCW/gS) Carbon balance (%)
Batch 0.46 ± 0.01 22.2 ± 0.09 87 ± 1.2 1.3 ± 0.05 1.38 ± 0.02 0.054 ± 0.001 91 ± 0.91
SSCC 0.05 0.05 18.2 ± 0.15 72 ± 0.6 0.9 ± 0.01 0.58 ± 0.03 0.032 ± 0.002 75 ± 0.55
SSCC 0.10 0.10 15 ± 0.4 82 ± 1 1.5 ± 0.04 0.81 ± 0.04 0.051 ± 0.002 91 ± 0.55
SSCC 0.15 0.15 10 ± 0.2 82 ± 2 1.5 ± 0.04 0.99 ± 0.02 0.064 ± 0.005 95 ± 1.3
SSCC 0.20 0.20 5.1 ± 0.2 77 ± 2 1.0 ± 0.04 1.04 ± 0.03 0.076 ± 0.003 96 ± 0.7
SSCC 0.30 0.30 1.8 ± 0.2 80 ± 2 0.53 ± 0.06 1.47 ± 0.02 0.087 ± 0.014 99 ± 0.5
  1. Average values and standard errors are shown from duplicate experiments for batch cultures and triplicate measurements during the steady state of continuous cultures
  2. D dilution rate, µ specific growth rate, YPS ethanol yield on sugars consumed as percentage of the maximum theoretical; YXS biomass yield on sugars consumed, QP volumetric ethanol productivity, qP specific ethanol productivity