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Table 2 Km, kcat, and specificity constant (kcat/Km) of the W182A mutant

From: Structural and functional characterization of a novel cold-active S-formylglutathione hydrolase (SfSFGH) homolog from Shewanella frigidimarina, a psychrophilic bacterium

Substrates Vmax (μM s−1) Km (mM) kcat (S−1) kcat/Km (s−1 mM−1)
p-NA 5.378 ± 0.268 1.393 ± 0.136 1.852 ± 0.092 1.3
p-NB 0.861 ± 0.035 0.303 ± 0.049 8.003 ± 0.326 26.4
p-NO 0.477 ± 0.023 0.495 ± 0.085 0.144 ± 0.007 0.3