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Table 2 Km, kcat, and specificity constant (kcat/Km) of the W182A mutant

From: Structural and functional characterization of a novel cold-active S-formylglutathione hydrolase (SfSFGH) homolog from Shewanella frigidimarina, a psychrophilic bacterium

Substrates

Vmax (μM s−1)

Km (mM)

kcat (S−1)

kcat/Km (s−1 mM−1)

p-NA

5.378 ± 0.268

1.393 ± 0.136

1.852 ± 0.092

1.3

p-NB

0.861 ± 0.035

0.303 ± 0.049

8.003 ± 0.326

26.4

p-NO

0.477 ± 0.023

0.495 ± 0.085

0.144 ± 0.007

0.3