Fig. 1From: Structural and functional characterization of a novel cold-active S-formylglutathione hydrolase (SfSFGH) homolog from Shewanella frigidimarina, a psychrophilic bacteriumEnzymatic activity of SfSFGH. a Substrate specificity was investigated using p-nitrophenyl esters with different acyl-chain lengths. b Effects of pH on enzymatic activity were studied from pH 3.0 to 10.0. Activity at the optimal pH was set as 100%. c Lineweaver–Burk plots showing the reciprocal of the velocity of SfSFGH versus the reciprocal of the substrate concentration. All experiments were performed in triplicateBack to article page