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Table 2 Specific activity, immobilization yield and activity recovery of GDH and FMO3

From: Drug metabolite synthesis by immobilized human FMO3 and whole cell catalysts

  Specific activitya (U/g) Immobilization yield (%) Activity recovery (%)
Before immobilization After immobilization
GDH 712 332 > 99 46
FMO3 462 173 > 99 37
  1. aOne unit (U) of enzyme activity is defined as the amount of enzyme required to release 1 μM of substrate per minute. The specific activity was determined at 35 °C in 50 mM Tris–HCl, pH 8.4. The immobilization yield and activity recovery were determined according to [13]