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Table 2 Specific activity, immobilization yield and activity recovery of GDH and FMO3

From: Drug metabolite synthesis by immobilized human FMO3 and whole cell catalysts

 

Specific activitya (U/g)

Immobilization yield (%)

Activity recovery (%)

Before immobilization

After immobilization

GDH

712

332

> 99

46

FMO3

462

173

> 99

37

  1. aOne unit (U) of enzyme activity is defined as the amount of enzyme required to release 1 μM of substrate per minute. The specific activity was determined at 35 °C in 50 mM Tris–HCl, pH 8.4. The immobilization yield and activity recovery were determined according to [13]