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Table 1 1H NMR chemical shift assignments for heparosan products from the engineered B. megaterium strain

From: Metabolic engineering of Bacillus megaterium for heparosan biosynthesis using Pasteurella multocida heparosan synthase, PmHS2

Proton

Chemical shift (ppm)

GlcNAc, CH3

2.05

GlcA H2

3.27

GlcA H3, H4 and GlcNAc H4

3.48–3.53

GlcA, H5

3.68

GlcNAc H2, H3, H5, H6

3.80–3.84

GlcA H1

4.62

GlcNAc, H1

5.57

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Microbial Cell Factories

ISSN: 1475-2859

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