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Fig. 8 | Microbial Cell Factories

Fig. 8

From: Metabolic engineering of Bacillus megaterium for heparosan biosynthesis using Pasteurella multocida heparosan synthase, PmHS2

Fig. 8

PAGE analysis using 15% resolving gel analysis of various B. megaterium heparosan products. S1 shows a ladder of heparin oligosaccharide standards of known molecular weights that were enzymatically prepared from bovine lung heparin [49]. S2 shows the band sizes using structurally defined oligosaccharides of degree of polymerization (dp)10 and (dp)20 [55]. Other lanes show the heparosan products, with the higher MW products in lanes B–D highlighted

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