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Table 1 Strains and plasmids used in this study

From: Engineering the pathway in Escherichia coli for the synthesis of medium-chain-length polyhydroxyalkanoates consisting of both even- and odd-chain monomers

Strains and plasmids Relevant genotype/property Source or references
Strains
 E. coli DH5a F, endA1, hsdR17, (rk, mk+), supE44, thi-1, λ, recA1, gyrA96, ΔlacU169 (Φ80 lacZ ΔM15) Laboratory stock
 E. coli LS5218 F+, fadR601, atoC512 (Const) Laboratory stock
 LZ05 E. coli LS5218 ΔptsG::FRT ΔtesB::FRT ΔyciA::FRT Zhuang et al. [23]
 LZ08 E. coli LS5218 ΔptsG::FRT ΔtesA::FRT ΔpflB::FRT ΔpoxB::FRT This study
Plasmids
 pBBR1MCS-2 lacPOZ mobRP4, low-copy-no. cloning vector; KmR Kovach et al. [37]
 pQQ05 pTrc99a derivative, yqeF and fadB from E. coli MG1655, phaJ1 Pa and phaC2 Pa from P. aeruginosa PAO1, ter from Treponema denticola Zhuang et al. [23]
 pZQ01 pBBR1MCS2-prpP; pBBR1MCS-2 derivative, prpP from R. eutropha H16 This study
 pZQ02 pBBR1MCS2-acs; pBBR1MCS-2 derivative, acs from E. coli MG1655 This study
 pZQ03 pBBR1MCS2-prpE; pBBR1MCS2-derivative, prpE from R. eutropha H16 This study
 pZQ04 pBBR1MCS2-pct; pBBR1MCS-2 derivative, pct from R. eutropha H16 This study
 pZQ05 pBBR1MCS2-prpP-acs; pBBR1MCS-2 derivative, prpP from R. eutropha H16 and acs from E. coli MG1655 This study
 pZQ06 pBBR1MCS2-prpP-prpE; pBBR1MCS2-derivative, prpP and prpE from R. eutropha H16 This study
 pZQ07 pBBR1MCS2-prpP-pct; pBBR1MCS2-derivative, prpP and pct from R. eutropha H16 This study