Table 1 Strains and plasmids used in this study

Strains

 E. coli DH5a

F⁻, endA1, hsdR17, (rk⁻, mk⁺), supE44, thi-1, λ⁻, recA1, gyrA96, ΔlacU169 (Φ80 lacZ ΔM15)

Laboratory stock

 E. coli LS5218

F⁺, fadR601, atoC512 (Const)

Laboratory stock

 LZ05

E. coli LS5218 ΔptsG::FRT ΔtesB::FRT ΔyciA::FRT

Zhuang et al. [23]

 LZ08

E. coli LS5218 ΔptsG::FRT ΔtesA::FRT ΔpflB::FRT ΔpoxB::FRT

This study

Plasmids

 pBBR1MCS-2

lacPOZ mobRP4, low-copy-no. cloning vector; Km^R

Kovach et al. [37]

 pQQ05

pTrc99a derivative, yqeF and fadB from E. coli MG1655, phaJ1 _Pa and phaC2 _Pa from P. aeruginosa PAO1, ter from Treponema denticola

Zhuang et al. [23]

 pZQ01

pBBR1MCS2-prpP; pBBR1MCS-2 derivative, prpP from R. eutropha H16

This study

 pZQ02

pBBR1MCS2-acs; pBBR1MCS-2 derivative, acs from E. coli MG1655

This study

 pZQ03

pBBR1MCS2-prpE; pBBR1MCS2-derivative, prpE from R. eutropha H16

This study

 pZQ04

pBBR1MCS2-pct; pBBR1MCS-2 derivative, pct from R. eutropha H16

This study

 pZQ05

pBBR1MCS2-prpP-acs; pBBR1MCS-2 derivative, prpP from R. eutropha H16 and acs from E. coli MG1655

This study

 pZQ06

pBBR1MCS2-prpP-prpE; pBBR1MCS2-derivative, prpP and prpE from R. eutropha H16

This study

 pZQ07

pBBR1MCS2-prpP-pct; pBBR1MCS2-derivative, prpP and pct from R. eutropha H16

This study