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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Engineering the pathway in Escherichia coli for the synthesis of medium-chain-length polyhydroxyalkanoates consisting of both even- and odd-chain monomers

Fig. 1

Schematic pathway for mcl-PHA biosynthesis consisting of odd-chain and even-chain monomers. Genes overexpressed or deleted and metabolic intermediates in the recombinant E. coli are marked. After every cycle, two carbons were added to the initial acyl-CoA thioester (indicated as Cn + 2) was generated. Dashed lines indicate multiple steps. Dashed lines without arrowheads connect acyl-CoA intermediates of different chain length. Genes in bold were expressed to obtain mcl-PHAs from the reversed β-oxidation cycle. Marked genes: ptsG, glucose-specific PTS permease, IIBC component; poxB: pyruvate oxidase; pflB: pyruvate formate-lyase; yqeF, thiolase; fadB, hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase; ter, trans-2-enoyl-CoA reductase from T. denticola; phaJ1Pa, (R)-specific enoyl-CoA hydratase from P. aeruginosa PAO1; phaC2Pa, polyhydroxyalkanoic acid synthase from P. aeruginosa PAO1; tesB/yciA, thioesterase; prpP, propionate permease; acs, acetyl-CoA synthase; prpE, propionyl-CoA synthase; pct, propionate-CoA transferase. The left yellow dotted box indicates the even-chain precursor (acetyl-CoA) supply module. Purple arrows indicate the odd-chain precursor (propionyl-CoA) supply module in the right yellow dotted box. Blue arrows indicate the reversed β-oxidation cycle module

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