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Table 2 Kinetic parameters for RAPc8 and indicated mutants

From: Development and application of a transcriptional sensor for detection of heterologous acrylic acid production in E. coli

RAPc8 enzyme variant Vmax (uM min−1) Km (mM) kcat (min−1) kcat/Km (mM−1 min−1)
WT 106.5 ± 6.1 7.3 ± 0.1 7096.3 ± 404.7 978.9 ± 63.3
A77T 66.7 ± 2.1 4.5 ± 0.2 4447.7 ± 139.2 996 ± 21.4
M203V 146.8 ± 16.7 7.2 ± 1.1 9784.7 ± 1113.3 1374.5 ± 70
A77T + M203V 64.7 ± 4.6 4.6 ± 0.4 4315 ± 308.4 935.7 ± 35.9
C60 176.3 ± 16.1 7.4 ± 1.3 11,754.3 ± 1073.3 1607.1 ± 143.5
  1. Values represent average ± SD (n = 3)