Table 2 Kinetic parameters for RAPc8 and indicated mutants
RAPc8 enzyme variant | Vmax (uM min−1) | Km (mM) | kcat (min−1) | kcat/Km (mM−1 min−1) |
|---|---|---|---|---|
WT | 106.5 ± 6.1 | 7.3 ± 0.1 | 7096.3 ± 404.7 | 978.9 ± 63.3 |
A77T | 66.7 ± 2.1 | 4.5 ± 0.2 | 4447.7 ± 139.2 | 996 ± 21.4 |
M203V | 146.8 ± 16.7 | 7.2 ± 1.1 | 9784.7 ± 1113.3 | 1374.5 ± 70 |
A77T + M203V | 64.7 ± 4.6 | 4.6 ± 0.4 | 4315 ± 308.4 | 935.7 ± 35.9 |
C60 | 176.3 ± 16.1 | 7.4 ± 1.3 | 11,754.3 ± 1073.3 | 1607.1 ± 143.5 |