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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Development and application of a transcriptional sensor for detection of heterologous acrylic acid production in E. coli

Fig. 7

(adapted from the crystal structure 2PLQ [28] and drawn using PyMOL [29])

Position of mutations present in selected RAPc8 C60 and C65 variants (magenta). Residues depicted in orange (E59, K134, C166) comprise the catalytic triad. M203 is shown in spheres to highlight packing against the distorted helix (residues 167–173 denoted in green) that likely undergoes structural changes to adapt to the loss of interactions when M203 is mutated to V with a smaller sidechain

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