Fig. 6From: Development and application of a transcriptional sensor for detection of heterologous acrylic acid production in E. coliFACS analysis indicating eGFP fluorescence of stably transduced E. coli (PyHCN-eGFP) cells expressing RAPc8 amidase (WT) or the inactive E142D and E142L mutants. Cells were treated with indicated concentrations of acrylic acid (AA, positive control) or acrylamide (ACM) for 2 h prior to analysisBack to article page