Table 5 primers used in this study
Primer | Sequence (5′–3′) and restriction site |
|---|---|
PalsSF | ACCGGTCGACAAAGGAGGGAAATCATGACAAAAGCAACAAAAG (SalI) |
PalsSR | ACCGGGATCCCTAGAGAGCTTTCGTTTTC (BamHI) |
PalsDF | ACCGGGATCCAAAGGAGGGAAATCATGAAACGAgAAAGCAAC (BamHI) |
PalsDR | ACCGGAATTCTTATTCAGGGCTTCCTTC (EcoRI) |
PbdhAF | ACCGAAGCTTAAAGGAGGGAAATCATGAAGGCAGCAAGATGG (HindIII) |
PbdhAR | ACCGGGATCCTTAGTTAGGTCTAACAAGG (BamHI) |
PalsSDF | ACCGCCCGGGAA AGGAGGGAAATCATGACAAAAGCAACAAAAG (SmaI) |
PalsSDR | ACCGGAGCTCTTATTCAGGGCTTCCTTC (SacI) |
PbutA1F | ACCGGAATTCATGAGCAAAGTTGCAATGGT (EcoRI) |
PbutA2R | ACCGAAGCTTCTAGTTGTAGAGCATGCCGC (HindIII) |
PbutA3R | TAGGCATTGACGGTGTGACCCTTGGCATCAATTGCACTGTCGAAATTAGC |
PbutA4F | GCTAATTTCGACAGTGCAATTGATGCCAAGGGTCACACCGTCAATGCCTA |
Pldh1F | ACCGGAATTCATGAAAGAAACCGTCGGT (EcoRI) |
Pldh2R | ACCGAAGCTTTTAGAAGAACTGCTTCTG (HindIII) |
Pldh3R | GTAGGATTGCGCGGGTGATGCGAGCCTTCGCAGTCAGCGTAGGTTCCCTT |
Pldh4F | AAGGGAACCTACGCTGACTGCGAAGGCTCGCATCACCCGCGCAATCCTAC |