Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method

Table 4 Comparison of titer and yield of AC and 2,3-BD produced by different strains through bioconversion in recent years

Strains	Bioconversion method	Substrates	Products	Titer (g L⁻¹)	Yield (mol mol⁻¹)	References
E. coli	Fed batch	DA	AC	39.4	0.83	[36]
E. coli BL21 (DE3)	Batch	meso-2,3-BD	(3R)-AC	86.7	0.94	[37]
E. coli BL21 (DE3)	Batch	meso-2,3-BD	(3S)-AC	36.7	0.88	[21]
E. coli BL21 (DE3)	Batch	(2R,3R)-2,3-BD	(3R)-AC	41.8	0.98	[21]
B. subtilis 168	Fed batch	2,3-BD	AC	91.8	0.78	[22]
E. coli	Batch	meso-2,3-BD	(3S)-AC	72.38	0.92	[38]
E. coli	Batch	meso-2,3-BD	(2S,3S)-2,3-BD	38.41	0.96	[38]
E. coli BL21 (DE3)	Fed batch	DA	(2S,3S)-2,3-BD	31.7	0.86	[39]
B. subtilis 168	Repeated batches	AC + formate	2,3-BD	115.4	0.96	[40]
B. subtilis 168	Batch	AC + glucose	2,3-BD	63.7	0.96	[40]
K. pneumoniae and B. subtilis 168	Two-step sequencing batch	Glucose	(3S)-AC	56.7	0.85	[41]
C. crenatumΔbutAΔldh SD	Repeated batches	Glucose	AC	76.9	0.67	This study
C. crenatumΔldh SDA	Repeated batches	Glucose	2,3-BD	88.8	0.80	This study

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