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Table 3 Specific enzyme activity assays of LDH and AR/BDH in crude cell extracts of recombinant C. crenatum strains

From: Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method

Strains LDH (U mg−1) AR/BDH (U mg−1)
C. crenatum WT 1.04 ± 0.03 0.055 ± 0.003/0.003 ± 0.001
C. crenatumΔldh 0.18 ± 0.01 nd
C. crenatumΔbutA nd 0.021 ± 0.003/0.0005 ± 0.001
C. crenatumΔbutAΔldh 0.13 ± 0.01 0.010 ± 0.002/0.0004 ± 0.001
  1. Using C. crenatum WT as positive control, 1% of recombinant C. crenatum was transferred to 50 mL LBG medium and incubated in shake flask at 180 r min−1, 30 °C for 12 h. The supernatant of the disrupted cells was taken to LDH and AR/BDH activity assay. The results of the specific enzyme activity assays are the mean ± standard of three replicates
  2. nd not detected