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Table 2 Fermentation kinetics by recombinant C. crenatum in shack flask

From: Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method

Strain Time (h) Glucose consumption (g L−1) Glucose uptake rate (g L−1 h−1) Diacetyl (g L−1) Acetoin (g L−1) 2,3-Butanediol (g L−1) l-Arginine (g L−1)
C. crenatum WT 120 110 ± 2 0.91 ± 0.02 nd nd nd 23.59 ± 1.7
C. crenatum S 120 36 ± 4 0.30 ± 0.03 2.17 ± 0.13 0.76 ± 0.12 0.45 ± 0.13 7.09 ± 0.70
C. crenatum D 120 78 ± 3 0.65 ± 0.02 nd nd nd 15.66 ± 1.31
C. crenatum A 120 73 ± 4 0.61 ± 0.03 nd nd nd 14.27 ± 1.45
C. crenatum SD 120 70 ± 3 0.58 ± 0.02 nd 13.59 ± 1.25 10.61 ± 1.33 8.74 ± 1.12
C. crenatum SDA 120 70 ± 6 0.58 ± 0.05 nd 9.43 ± 1.50 15.16 ± 1.31 7.83 ± 0.84
  1. Using C. crenatum WT as positive control, 10% recombinant C. crenatum was transferred to the fermentation medium containing 120 g L−1 glucose for shake flask fermentation (120 g L−1 glucose was used as substrate). The titer of DA, AC, 2,3-BD and l-arginine were determined after 120 h of fermentation. The results of the fermentation parameters are the mean ± standard of three biological replicates
  2. nd not detected