Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method

Table 2 Fermentation kinetics by recombinant C. crenatum in shack flask

Strain	Time (h)	Glucose consumption (g L⁻¹)	Glucose uptake rate (g L⁻¹ h⁻¹)	Diacetyl (g L⁻¹)	Acetoin (g L⁻¹)	2,3-Butanediol (g L⁻¹)	l-Arginine (g L⁻¹)
C. crenatum WT	120	110 ± 2	0.91 ± 0.02	nd	nd	nd	23.59 ± 1.7
C. crenatum S	120	36 ± 4	0.30 ± 0.03	2.17 ± 0.13	0.76 ± 0.12	0.45 ± 0.13	7.09 ± 0.70
C. crenatum D	120	78 ± 3	0.65 ± 0.02	nd	nd	nd	15.66 ± 1.31
C. crenatum A	120	73 ± 4	0.61 ± 0.03	nd	nd	nd	14.27 ± 1.45
C. crenatum SD	120	70 ± 3	0.58 ± 0.02	nd	13.59 ± 1.25	10.61 ± 1.33	8.74 ± 1.12
C. crenatum SDA	120	70 ± 6	0.58 ± 0.05	nd	9.43 ± 1.50	15.16 ± 1.31	7.83 ± 0.84

Using C. crenatum WT as positive control, 10% recombinant C. crenatum was transferred to the fermentation medium containing 120 g L⁻¹ glucose for shake flask fermentation (120 g L⁻¹ glucose was used as substrate). The titer of DA, AC, 2,3-BD and l-arginine were determined after 120 h of fermentation. The results of the fermentation parameters are the mean ± standard of three biological replicates
nd not detected

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