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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method

Fig. 2

Resting cell bioconversion analysis of C. crenatum and the recombinant strains in shack flask. Recombinant C. crenatum (100 µL) was transferred to 10 mL LBG medium and incubated for approximately 12 h at 30 °C, 180 r min−1. The cultured bacterial solution (3 mL) was transferred into 30 mL of fermentation medium at the same culture condition for 24 h. The cultured cells were harvested by centrifugation at 8000 r min−1 for 10 min and then resuspended in resting cell bioconversion medium (containing 100 g L−1 glucose) for resting cell bioconversion. Formed metabolites and organic acid during the bioconversion of resting cells are shown. The results of resting cell bioconversion kinetics are shown as the mean ± standard of three replicates. +: overexpression, Δ: knockout

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