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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Shaping the lipid composition of bacterial membranes for membrane protein production

Fig. 4

Coomassie Brilliant Blue stained 10% SDS-PAGE of the purification of HlyB from E. coli C41(DE3)∆(ompF-acrAB) employing different detergent-combinations for solubilization (first detergent) and subsequent IMAC (second detergent). (1) Fos-choline 14/DDM, (2) DDM/DDM, (3) TX-100/DDM. Shown are the pooled eluate fractions of equal volume. Independent expressions and purifications were performed two times with similar results

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