Fig. 3

Analysis of gene expression in Corynebacterium glutamicum. a Northern blot analysis of total RNA isolated from non-induced (n.i.) and IPTG-induced cells of C. glutamicum (harvested 2 h, 5 h and 18 h after induction). Total RNA (5 µg each lane) was hybridized to a rosB-specific DIG-labelled probe. The expected rosB-containing RNA transcript of 2.5 knu is indicated by an arrow. b SDS-PAGE analysis of cell-free extracts prepared from non-induced (n.i.) and IPTG-induced cells of C. glutamicum strains (harvested 2 h and 6 h after induction). The samples (10 µg of total protein) were loaded onto the gel which after electrophoresis was stained with Coomassie Brilliant Blue R-250. Red boxes indicate the expected molecular masses for the gene products of rosB (29 kDa), rosA (38 kDa) and RFK (17.6 kDa), as well as the excised gel fragments used for MALDI-TOF MS analysis of RosB, RosA and RFK