Skip to main content

Advertisement

Table 3 Strains and plasmids used in this work

From: Fructose metabolism in Chromohalobacter salexigens: interplay between the Embden–Meyerhof–Parnas and Entner–Doudoroff pathways

Strains Relevant genotype/use Reference or source
Chromohalobacter salexigens CHR61 Spontaneous Rfr mutant of DSM 3043 Cánovas et al. [54]
E. coli Top10F’ Host for pRSETB and pRSETC plasmids propagation Invitrogen
E. coli BL21(DE3) ΔpatZ Host for heterologous protein expression; F ompT hsdSB(rB- mB-) gal dcm λ(DE3) tonA ΔpatZ Castaño-Cerezo et al. [53]
E. coli JW4191-1 fbp-779::kan Baba et al. [51]
E. coli RL257 ΔpfkA ΔpfkB Lovingshimer et al. [52]
Plasmids Cloned region Source
pRSETB   Life Technologies
pRSETBCsal0639p   This work
pRSETC   Life Technologies
pRSETCCsal0931p   This work
pRSETCCsal2647p   This work