Table 3 Strains and plasmids used in this work
Strains | Relevant genotype/use | Reference or source |
|---|---|---|
Chromohalobacter salexigens CHR61 | Spontaneous Rfr mutant of DSM 3043 | Cánovas et al. [54] |
E. coli Top10F’ | Host for pRSETB and pRSETC plasmids propagation | Invitrogen |
E. coli BL21(DE3) ΔpatZ | Host for heterologous protein expression; F− ompT hsdSB(rB- mB-) gal dcm λ(DE3) tonA ΔpatZ | Castaño-Cerezo et al. [53] |
E. coli JW4191-1 | fbp-779::kan | Baba et al. [51] |
E. coli RL257 | ΔpfkA ΔpfkB | Lovingshimer et al. [52] |
Plasmids | Cloned region | Source |
pRSETB | Life Technologies | |
pRSETBCsal0639p | This work | |
pRSETC | Life Technologies | |
pRSETCCsal0931p | This work | |
pRSETCCsal2647p | This work |