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Table 2 Percentage of 13C-labelling at each of the carbon atoms of ectoines synthesized by C. salexigens using either [1-13C]-fructose or [1-13C]-glucose as the sole carbon source

From: Fructose metabolism in Chromohalobacter salexigens: interplay between the Embden–Meyerhof–Parnas and Entner–Doudoroff pathways

 

Labelled position

[1-13C]-fructose

[1-13C]-glucose

CH3 (methyl)

8.30 ± 1.76

1.17 ± 0.10

C2

1.65 ± 0.14

1.16 ± 0.28

COOH

17.14 ± 0.06

28.65 ± 0.85

C4

3.59 ± 0.70

1.16 ± 0.37

C5

7.23 ± 1.76

1.02 ± 0.22

C6

10.04 ± 1.66

14.36 ± 2.11

  1. C. salexigens were grown in M63 minimal medium supplemented with 0.75 M NaCl, using either labelled fructose or glucose as the sole carbon source. Fructose cultures were carried out in triplicate; glucose cultures were carried out in duplicate
  2. In order to quantify labelling, percentage of labelling were estimated for each sample analyzed independently from quantitative 1H-NMR and 13C-NMR spectra. Estimations for ectoine and hydroxyectoine were carried out separately for each condition
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Microbial Cell Factories

ISSN: 1475-2859

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