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Table 2 Percentage of 13C-labelling at each of the carbon atoms of ectoines synthesized by C. salexigens using either [1-13C]-fructose or [1-13C]-glucose as the sole carbon source

From: Fructose metabolism in Chromohalobacter salexigens: interplay between the Embden–Meyerhof–Parnas and Entner–Doudoroff pathways

  Labelled position [1-13C]-fructose [1-13C]-glucose
CH3 (methyl) 8.30 ± 1.76 1.17 ± 0.10
C2 1.65 ± 0.14 1.16 ± 0.28
COOH 17.14 ± 0.06 28.65 ± 0.85
C4 3.59 ± 0.70 1.16 ± 0.37
C5 7.23 ± 1.76 1.02 ± 0.22
C6 10.04 ± 1.66 14.36 ± 2.11
  1. C. salexigens were grown in M63 minimal medium supplemented with 0.75 M NaCl, using either labelled fructose or glucose as the sole carbon source. Fructose cultures were carried out in triplicate; glucose cultures were carried out in duplicate
  2. In order to quantify labelling, percentage of labelling were estimated for each sample analyzed independently from quantitative 1H-NMR and 13C-NMR spectra. Estimations for ectoine and hydroxyectoine were carried out separately for each condition