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Table 1 Enzyme activities detected by spectrophotometric methods in the three purified proteins expressed in E. coli BL21 (DE3) ΔpatZ from the corresponding heterologous genes of C. salexigens

From: Fructose metabolism in Chromohalobacter salexigens: interplay between the Embden–Meyerhof–Parnas and Entner–Doudoroff pathways

Enzyme activity

Csal2647

Csal0931

Csal0639

Frk

0.05 ± 0.02

2.71 ± 0.75

ND

1-Pfk

1.12 ± 0.32

ND

ND

  1. Values shown are the average and standard deviation of at least two biological replicates and three technical replicates, and correspond to units of enzyme activity per milligram of protein. Proteins were also assayed for the following enzyme activities (although no detectable activity was observed)
  2. Frk: fructokinase; 1-Pfk: 1-phosphofructokinase; 6-Pfk: 6-phosphofructokinase; Glk: glucokinase; Pfp: pyrophosphate dependent 6-phosphofructokinase; ND: not detected
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Microbial Cell Factories

ISSN: 1475-2859

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