Fig. 6

Growth experiments to test functional complementation of fructose bisphosphatase and phosphofructokinase mutations by the csal15354 gene. Cultures were grown in M9 containing glycerol or mannose as sole carbon sources as indicated, plus kanamycin. a Host strain was E. coli JW4191 (Δfbp); b host strain was E. coli RL257 (ΔpfkA ΔpfkB). The strains carried the empty cloning vector pSMART-HCKan (ev) or the csal1534 gene inserted into pSMART-HCKan. The results are the averages of experimental replicates. Where not visible, the experimental variability was less than the size of the symbols