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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Fructose metabolism in Chromohalobacter salexigens: interplay between the Embden–Meyerhof–Parnas and Entner–Doudoroff pathways

Fig. 5

13C-NMR spectra of intracellular extracts of a [1-13C]-fructose and of b [1-13C]-glucose grown cultures. M63 minimal medium with 0.75 M NaCl and 20 mM of the isotopically labeled carbon source was used. Signals corresponding to labeled carboxylic carbon (COOH), C4, C5, C6 and methyl group (CH3) from ectoine (E) and hydroxyectoine (H) are noted. The carbon backbone schemes of ectoines are coloured according to the experimental percentage of 13C isotopic abundance at each carbon position of ectoine and hydroxyectoine. Relative labeling for each carbon atom is indicated by the color scale at the right of b. Note that largest differences between carbon sources in percentage of labeling are found for C5 and CH3 positions, the last one being taken as an indicator of EMP flux contribution to the synthesis of ectoines

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