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Table 3 Strains and plasmids used in this study

From: Metabolic engineering for efficient supply of acetyl-CoA from different carbon sources in Escherichia coli

Strain/plasmid Description Reference
Strains
 E. coli DH5α FendA1 glnV44 thi1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZM15(lacZYA-argF)U169, hsdR17(r K m + K ), λ Invitrogen
 E. coli BW25113 lacIqrrnBT14lacZWJ16hsdR514araBADAH33rhaBADLD78 Invitrogen
 N00 E. coli BW25113, ∆argB,argA This study
 N01 E. coli BW25113, ∆argB,argA, ∆ptsG::glk,galR::zglf This study
 N02 E. coli BW25113, ∆argB,argA, ∆ptsG::glk,galR::zglf, ∆poxB::acs This study
 N03 E. coli BW25113, ∆argB,argA, ∆ptsG::glk,galR::zglf, ∆poxB::acs, ∆ldhA This study
 N04 E. coli BW25113, ∆argB,argA, ∆ptsG::glk,galR::zglf, ∆poxB::acs, ∆ldhA, ∆pta This study
 N05 E. coli BW25113, ∆argB,argA, ∆ptsG::glk,galR::zglf, ∆poxB::acs, ∆ldhA, ∆gltA This study
 N06 E. coli BW25113, ∆argB,argA, ∆fadR, PfadD::PCPA1 This study
Plasmids
 pRB1 s RSF1030 origin, Pbad promoter, StrR Our lab
 pSB1a pSC101 origin, Pbad promoter, AmpR Our lab
 pN01 argA from E. coli cloned into pRB1 s This study
 pN02 argA from Pseudomonas aeruginose cloned into pRB1 s This study
 pN03 argA from Xanthomonas campestris cloned into pRB1 s This study
 pN05 argA from Corynebacterium glutamicum cloned into pRB1 s This study
 pN06 argA from Streptomyces coelicolor cloned into pRB1 s This study
 pN08 argA from Mycobacterium tuberculosis cloned into pRB1 s This study
 pN14 argA from Thermus thermophilus cloned into pRB1 s This study
 pN18 argA from Meiothermus ruber cloned into pRB1 s This study
 pN19 argA from Kitasatospora setae cloned into pRB1 s This study
 pN20 argA from Deinococcus deserti cloned into pRB1 s This study
 pAcs acs from E. coli cloned into pSB1a This study
 pAck ackA-pta from E. coli cloned into pSB1a This study