Table 3 Strains and plasmids used in this study

Strains

 E. coli DH5α

F⁻endA1 glnV44 thi⁻1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZ ∆M15 ∆(lacZYA-argF)U169, hsdR17(r ⁻ _K m ⁺ _K ), λ⁻

Invitrogen

 E. coli BW25113

lacI^qrrnB_T14∆lacZ_WJ16hsdR514∆araBAD_AH33∆rhaBAD_LD78

Invitrogen

 N00

E. coli BW25113, ∆argB, ∆argA

This study

 N01

E. coli BW25113, ∆argB, ∆argA, ∆ptsG::glk, ∆galR::zglf

This study

 N02

E. coli BW25113, ∆argB, ∆argA, ∆ptsG::glk, ∆galR::zglf, ∆poxB::acs

This study

 N03

E. coli BW25113, ∆argB, ∆argA, ∆ptsG::glk, ∆galR::zglf, ∆poxB::acs, ∆ldhA

This study

 N04

E. coli BW25113, ∆argB, ∆argA, ∆ptsG::glk, ∆galR::zglf, ∆poxB::acs, ∆ldhA, ∆pta

This study

 N05

E. coli BW25113, ∆argB, ∆argA, ∆ptsG::glk, ∆galR::zglf, ∆poxB::acs, ∆ldhA, ∆gltA

This study

 N06

E. coli BW25113, ∆argB, ∆argA, ∆fadR, P_fadD::P_CPA1

This study

Plasmids

 pRB1 s

RSF1030 origin, Pbad promoter, Str^R

Our lab

 pSB1a

pSC101 origin, Pbad promoter, Amp^R

Our lab

 pN01

argA from E. coli cloned into pRB1 s

This study

 pN02

argA from Pseudomonas aeruginose cloned into pRB1 s

This study

 pN03

argA from Xanthomonas campestris cloned into pRB1 s

This study

 pN05

argA from Corynebacterium glutamicum cloned into pRB1 s

This study

 pN06

argA from Streptomyces coelicolor cloned into pRB1 s

This study

 pN08

argA from Mycobacterium tuberculosis cloned into pRB1 s

This study

 pN14

argA from Thermus thermophilus cloned into pRB1 s

This study

 pN18

argA from Meiothermus ruber cloned into pRB1 s

This study

 pN19

argA from Kitasatospora setae cloned into pRB1 s

This study

 pN20

argA from Deinococcus deserti cloned into pRB1 s

This study

 pAcs

acs from E. coli cloned into pSB1a

This study

 pAck

ackA-pta from E. coli cloned into pSB1a

This study