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Table 3 Changes of different parameters during fermentation in 5 L fermenter of BL Δupp and BL ΔF

From: Optimization of alkaline protease production by rational deletion of sporulation related genes in Bacillus licheniformis

Time/h

BL Δupp

BL ΔF

VC/cfu/mL

DO2/%

pH

RS/%

VC/cfu/mL

DO2/%

pH

RS/%

0

5.71 × 104

90

7.28

23.67

6.33 × 104

90

7.26

24.18

12

4.95 × 108

53.4

7.13

19.70

5.86 × 108

50.7

7.16

20.03

24

1.23 × 1010

32.0

6.97

16.76

1.44 × 1010

33.6

7.23

16.85

36

1.87 × 1010

37.9

7.11

16.64

2.09 × 1010

34.9

7.19

16.54

48

3.62 × 1010

35.7

7.10

15.89

3.13 × 1010

36.3

7.15

16.16

60

3.47 × 1010

36.1

7.17

16.12

2.95 × 1010

35.5

7.19

17.04

72

1.96 × 1010

37.4

7.09

16.06

2.46 × 1010

36.2

7.11

16.51

  1. VC viable counts, DO2 dissolved oxygen, RS represents reducing sugar