Table 1 Strains and plasmids used in the study
Strain or plasmid | Characteristics or purpose | Reference |
|---|---|---|
Strains | ||
 E. coli EC135 | Knockout vectors construction | Chinese Academy of Science |
 E. coli EC135 pM.Bam | Plasmid DNA methylation modification | Chinese Academy of Science |
 B. licheniformis 2709 Δupp (BL Δupp) | Parent host | This work |
 B. licheniformis ΔsigF (BL ΔF) | ΔsigF, sigF gene deletion | This work |
 B. licheniformis ΔsigE (BL ΔE) | ΔsigE, sig E gene deletion | This work |
 B. licheniformis Δspo0A (BL Δ0A) | Δspo0A, spo0A gene deletion | This work |
 B. licheniformis CsigF (BL CF) | sigF gene complementation | This work |
 B. licheniformis CsigE (BL CE) | sigE gene complementation | This work |
 B. licheniformis Cspo0A (BL C0A) | spo0A gene complementation | This work |
Plasmids | ||
 pWH1520 | Shuttle expression vector, Ampr (E. coli) and Tetr (Bacillus): MCS | Nankai University |
 pWHU | pWH1520, upr T gene, cas9 gene | This work |
 pWHF | Knockout vector, spo IIAC gene deletion | This work |
 pWHE | Knockout vector, sig E gene deletion | This work |
 pWHA | Knockout vector, spo0A gene deletion | This work |
 pWHCF | Backcrossed vector, spo IIAC gene complementation | This work |
 pWHCE | Backcrossed vector, sig E gene complementation | This work |
 pWHCA | Backcrossed vector, spo0A gene complementation | This work |