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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Optimization of alkaline protease production by rational deletion of sporulation related genes in Bacillus licheniformis

Fig. 2

Confirmation of disruption of spoIIAC, spo0A and sigE. The DNA sequencing, alignment result of the deletion-carrying mutant and agarose gel electrophoresis; a spoIIAC deletion, losing 756 bp; b spo0A deletion, losing 459 bp; c sigE gene deletion, losing 612 bp. M: nucleic acid marker; NC: The negative control was obtained by colony PCR of the wild-strain

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