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Table 2 PBD matrix for 22 medium components with coded values and their corresponding net threonine produced and cell population

From: Optimized medium via statistical approach enhanced threonine production by Pediococcus pentosaceus TL-3 isolated from Malaysian food

Std run

A

B

C

D

E

F

G

H

J

K

L

M

N

O

P

Q

R

S

T

U

V

W

X

Net threonine produced (mg/L)

Cell population (log CFU/mL)

1

1

1

1

1

1

− 1

1

− 1

1

1

− 1

− 1

1

1

− 1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

29.95 ± 0.99F

8.50 ± 0.02N

2

− 1

1

1

1

1

1

− 1

1

− 1

1

1

− 1

− 1

1

1

− 1

− 1

1

− 1

1

− 1

− 1

− 1

0.00 ± 0.00N

9.44 ± 0.03B

3

− 1

− 1

1

1

1

1

1

− 1

1

− 1

1

1

− 1

− 1

1

1

− 1

− 1

1

− 1

1

− 1

− 1

12.39 ± 0.29K

9.20 ± 0.02EF

4

− 1

− 1

− 1

1

1

1

1

1

− 1

1

− 1

1

1

− 1

− 1

1

1

− 1

− 1

1

− 1

1

− 1

42.84 ± 1.23C

8.89 ± 0.03K

5

− 1

− 1

− 1

− 1

1

1

1

1

1

− 1

1

− 1

1

1

− 1

− 1

1

1

− 1

− 1

1

− 1

1

56.82 ± 1.44B

9.24 ± 0.01DE

6

1

− 1

− 1

− 1

− 1

1

1

1

1

1

− 1

1

− 1

1

1

− 1

− 1

1

1

− 1

− 1

1

− 1

39.72 ± 0.96D

9.03 ± 0.02H

7

− 1

1

− 1

− 1

− 1

− 1

1

1

1

1

1

− 1

1

− 1

1

1

− 1

− 1

1

1

− 1

− 1

1

27.97 ± 0.79G

8.59 ± 0.01M

8

1

− 1

1

− 1

− 1

− 1

− 1

1

1

1

1

1

− 1

1

− 1

1

1

− 1

− 1

1

1

− 1

− 1

14.69 ± 0.46J

9.00 ± 0.01HI

9

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

1

1

1

− 1

1

− 1

1

1

− 1

− 1

1

1

− 1

0.00 ± 0.00N

7.45 ± 0.00Q

10

− 1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

1

1

1

− 1

1

− 1

1

1

− 1

− 1

1

1

0.00 ± 0.00N

7.91 ± 0.01O

11

1

− 1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

1

1

1

− 1

1

− 1

1

1

− 1

− 1

1

4.30 ± 0.05M

8.97 ± 0.03IJ

12

1

1

− 1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

1

1

1

− 1

1

− 1

1

1

− 1

− 1

7.60 ± 0.15L

8.72 ± 0.01L

13

− 1

1

1

− 1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

1

1

1

− 1

1

− 1

1

1

− 1

41.26 ± 0.89CD

9.24 ± 0.01DE

14

− 1

− 1

1

1

− 1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

1

1

1

− 1

1

− 1

1

1

14.06 ± 0.28JK

8.60 ± 0.00M

15

1

− 1

− 1

1

1

− 1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

1

1

1

− 1

1

− 1

1

16.84 ± 0.35I

9.28 ± 0.01D

16

1

1

− 1

− 1

1

1

− 1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

1

1

1

− 1

1

− 1

8.71 ± 0.23L

9.10 ± 0.01G

17

− 1

1

1

− 1

− 1

1

1

− 1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

1

1

1

− 1

1

30.76 ± 0.73F

8.97 ± 0.01IJ

18

1

− 1

1

1

− 1

− 1

1

1

− 1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

1

1

1

− 1

17.66 ± 0.25HI

8.51 ± 0.01N

19

− 1

1

− 1

1

1

− 1

− 1

1

1

− 1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

1

1

1

17.98 ± 0.50HI

8.90 ± 0.02K

20

1

− 1

1

− 1

1

1

− 1

− 1

1

1

− 1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

1

1

14.04 ± 0.22JK

8.93 ± 0.02JK

21

1

1

− 1

1

− 1

1

1

− 1

− 1

1

1

− 1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

1

8.36 ± 0.28L

9.16 ± 0.01F

22

1

1

1

− 1

1

− 1

1

1

− 1

− 1

1

1

− 1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

1

35.50 ± 0.71E

9.38 ± 0.01C

23

1

1

1

1

− 1

1

− 1

1

1

− 1

− 1

1

1

− 1

− 1

1

− 1

1

− 1

− 1

− 1

− 1

1

19.37 ± 0.55H

8.99 ± 0.01HI

24

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

− 1

0.00 ± 0.00N

7.73 ± 0.02P

MRS

1

− 1

− 1

− 1

− 1

1

1

1

1

− 1

− 1

− 1

− 1

1

1

1

1

1

− 1

− 1

− 1

− 1

− 1

63.30 ± 2.10A

9.57 ± 0.01A

  1. Values are mean ± SEM, n = 3. Mean ± SEM within the same column that share a common superscript are not significantly different (p > 0.05)