Optimized medium via statistical approach enhanced threonine production by Pediococcus pentosaceus TL-3 isolated from Malaysian food

Table 10 CCD matrix with coded value and their corresponding experimental and predicted net threonine produced by P. pentosaceus TL-3

Std run	A	B	C	D	Net threonine produced (mg/L)
Std run	A	B	C	D	Experimental	Predicted^a
1	− 1	− 1	− 1	− 1	71.90 ± 0.19^L	71.79
2	1	− 1	− 1	− 1	75.28 ± 0.19^K	75.95
3	− 1	1	− 1	− 1	76.98 ± 0.24^K	77.71
4	1	1	− 1	− 1	84.67 ± 0.50^H	83.27
5	− 1	− 1	1	− 1	79.72 ± 0.87^IJ	80.07
6	1	− 1	1	− 1	85.62 ± 0.20^H	83.83
7	− 1	1	1	− 1	89.11 ± 0.41^G	92.59
8	1	1	1	− 1	98.25 ± 0.41^D	97.75
9	− 1	− 1	− 1	1	75.87 ± 0.30^K	75.07
10	1	− 1	− 1	1	81.66 ± 0.09^I	79.19
11	− 1	1	− 1	1	82.09 ± 0.06^I	84.91
12	1	1	− 1	1	92.07 ± 0.42^F	90.43
13	− 1	− 1	1	1	77.48 ± 0.11^JK	79.87
14	1	− 1	1	1	85.61 ± 0.09^H	83.59
15	− 1	1	1	1	98.27 ± 0.29^D	96.31
16	1	1	1	1	100.29 ± 0.17^D	101.43
17	− 2	0	0	0	84.58 ± 0.46^H	80.99
18	2	0	0	0	86.42 ± 0.26^H	90.27
19	0	− 2	0	0	67.87 ± 0.13^M	69.59
20	0	2	0	0	94.80 ± 0.24^E	93.35
21	0	0	− 2	0	64.24 ± 0.15^N	65.19
22	0	0	2	0	85.14 ± 0.16^H	84.47
23	0	0	0	− 2	93.91 ± 0.13^EF	93.03
24	0	0	0	2	98.88 ± 0.22^D	99.99
25	0	0	0	0	118.84 ± 1.58^C	121.27
26	0	0	0	0	118.93 ± 0.79^C	121.27
27	0	0	0	0	124.95 ± 2.26^A	121.27
28	0	0	0	0	122.01 ± 2.60^AB	121.27
29	0	0	0	0	120.24 ± 1.55^BC	121.27
30	0	0	0	0	122.65 ± 1.25^AB	121.27
MRS	–	–	–	–	60.47 ± 0.72^O	–

Values are mean ± standard error of mean (SEM), n = 3. Mean ± SEM within the same column that share different superscript are significantly different (p < 0.05)
^aPredicted net threonine produced was calculated based on Eq. 3

ISSN: 1475-2859