Whole transcriptome analysis and gene deletion to understand the chloramphenicol resistance mechanism and develop a screening method for homologous recombination in Myxococcus xanthus

Table 5 Plasmids used in this study

Name	Description	Reference or source
pBJ113	pBR322 ori,GalK; Kan^R	Julien et al. [32]
pR6K-Cm-ccdB	R6K ori, Cm^R,	Wang et al. [33]
pBJ113-5479Up	Kan^R, one homologous arm of MXAN_5479 flanked by EcoRI + XbaI	This study
pBJ113-5479	KanR, two homologous arms of MXAN_5479 flanked by EcoRI + HindIII	This study
pBJ113-2566Up	KanR, one homologous arm of MXAN_2566 flanked by EcoRI + XbaI	This study
pBJ113-2566	KanR, two homologous arms of MXAN_2566 flanked by EcoRI + HindIII	This study
pR6K-Cm	CmR, R6K ori which can not replicate in normal E.coli	This study
pR6K-Cm-11	CmR, contains 1.9-kb homologous arm located in TA gene cluster	This study
pR6K-Cm-14	CmR, contains 1.6-kb homologous arm located in yellow pigment gene cluster	This study

ISSN: 1475-2859