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Table 5 Plasmids used in this study

From: Whole transcriptome analysis and gene deletion to understand the chloramphenicol resistance mechanism and develop a screening method for homologous recombination in Myxococcus xanthus

Name

Description

Reference or source

pBJ113

pBR322 ori,GalK; KanR

Julien et al. [32]

pR6K-Cm-ccdB

R6K ori, CmR,

Wang et al. [33]

pBJ113-5479Up

KanR, one homologous arm of MXAN_5479 flanked by EcoRI + XbaI

This study

pBJ113-5479

KanR, two homologous arms of MXAN_5479 flanked by EcoRI + HindIII

This study

pBJ113-2566Up

KanR, one homologous arm of MXAN_2566 flanked by EcoRI + XbaI

This study

pBJ113-2566

KanR, two homologous arms of MXAN_2566 flanked by EcoRI + HindIII

This study

pR6K-Cm

CmR, R6K ori which can not replicate in normal E.coli

This study

pR6K-Cm-11

CmR, contains 1.9-kb homologous arm located in TA gene cluster

This study

pR6K-Cm-14

CmR, contains 1.6-kb homologous arm located in yellow pigment gene cluster

This study