Genotype | Reference | |
---|---|---|
Strains | ||
E. coli TOP10 | F–mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu) 7697 galUgalKrpsL (StrR) endA1 nupG | Life technologies |
E. coli GBdir-pir116 | DH10B, fhuA::IS2, ΔybcC, ΔrecET, pir116) with an arabinose-inducible ETγA operon (full-length recE, recT, redγ and recA), and a copy-up pir116 gene in chromosome); this allows the replication of R6K plasmids in high copy number | Wang et al. [33] |
M. xanthus | ||
DK1622 | Wild type | Kaiser [34] |
DK-5479 | DK1622 ΔMXAN_5479 | This study |
DK-2566 | DK1622 ΔMXAN_2566 | This study |
DK-Cm-11 | DK-2566∷pR6K-Cm-11 | This study |
DK-Cm-14 | DK-2566∷pR6K-Cm-14 | This study |