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Table 4 Strains used in this study

From: Whole transcriptome analysis and gene deletion to understand the chloramphenicol resistance mechanism and develop a screening method for homologous recombination in Myxococcus xanthus

  Genotype Reference
Strains
 E. coli TOP10 F–mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu) 7697 galUgalKrpsL (StrR) endA1 nupG Life technologies
 E. coli GBdir-pir116 DH10B, fhuA::IS2, ΔybcC, ΔrecET, pir116) with an arabinose-inducible ETγA operon (full-length recE, recT, redγ and recA), and a copy-up pir116 gene in chromosome); this allows the replication of R6K plasmids in high copy number Wang et al. [33]
M. xanthus
 DK1622 Wild type Kaiser [34]
 DK-5479 DK1622 ΔMXAN_5479 This study
 DK-2566 DK1622 ΔMXAN_2566 This study
 DK-Cm-11 DK-2566pR6K-Cm-11 This study
 DK-Cm-14 DK-2566pR6K-Cm-14 This study