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Table 4 Strains used in this study

From: Whole transcriptome analysis and gene deletion to understand the chloramphenicol resistance mechanism and develop a screening method for homologous recombination in Myxococcus xanthus

 

Genotype

Reference

Strains

 E. coli TOP10

F–mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu) 7697 galUgalKrpsL (StrR) endA1 nupG

Life technologies

 E. coli GBdir-pir116

DH10B, fhuA::IS2, ΔybcC, ΔrecET, pir116) with an arabinose-inducible ETγA operon (full-length recE, recT, redγ and recA), and a copy-up pir116 gene in chromosome); this allows the replication of R6K plasmids in high copy number

Wang et al. [33]

M. xanthus

 DK1622

Wild type

Kaiser [34]

 DK-5479

DK1622 ΔMXAN_5479

This study

 DK-2566

DK1622 ΔMXAN_2566

This study

 DK-Cm-11

DK-2566pR6K-Cm-11

This study

 DK-Cm-14

DK-2566pR6K-Cm-14

This study