Table 1 Strains, plasmids, and transformants used in the present study
From: Reconstruction of metabolic pathway for isobutanol production in Escherichia coli
Strains or plasmids | Genotype | Source or reference |
|---|---|---|
Strains | ||
NovaBlue | endA1 hsdR17 (rK12−mK12+) supE44 thi-I gyrA96 relA1 lac recA1/F’ [proAB+ lacIq ZΔM15::Tn10 (Tetr)]; used for gene cloning | Novagen |
ATCC 700926 | MG1655 | American Type Culture Collection |
CFTi2 | ATCC 700926 | ATCC |
CFTi3 | CFTi2Δpgi | This study |
CFTi4 | CFTi3ΔgntR | This study |
CFTi5 | CFTi4Δgnd | This study |
CFTi6 | CFTi5ΔpflB | This study |
CFTi9 | CFTi6ΔldhA | This study |
CFTi10 | CFTi7Δpta | This study |
CFTi2a | CFTi2 harboring pTka | This study |
CFTi3a | CFTi3 harboring pTka | This study |
CFTi4a | CFTi4 harboring pTka | This study |
CFTi5a | CFTi5 harboring pTka | This study |
CFTi6a | CFTi6 harboring pTka | This study |
CFTi9a | CFTi9 harboring pTka | This study |
CFTi10a | CFTi10 harboring pTka | This study |
CFTi21 | CFTi2a harboring p23SCD | This study |
CFTi31 | CFTi3a harboring p23SCD | This study |
CFTi41 | CFTi4a harboring p23SCD | This study |
CFTi51 | CFTi5a harboring p23SCD | This study |
CFTi61 | CFTi6a harboring p23SCD | This study |
CFTi91 | CFTi9a harboring p23SCD | This study |
CFTi101 | CFTi10a harboring p23SCD | This study |
CFTi91co | CFTi91 harboring pSAK | This study |
CFTi91zp | CFTi91 harboring pSzp | This study |
CFTi91zpee | CFTi91 harboring pSzpee | This study |
Plasmids | ||
pTrcHis B | Ptrc, pBR322 ori, Ampr | Life Technologies |
pZE12MCS | PLlacO1, colE1 ori, Ampr | Expressys |
pZA23MCS | PAlacO1, p15A ori, Kmr | Expressys |
pZA23trc | Ptrc, p15A ori, Kmr | This study |
pSAK | PAlacO1, SC101 ori, Cmr | [23] |
pZka | pZE12MCS containing kivd and adhAfrom Lactococcus lactis | This study |
pTka | pTrcHis B containing kivd and adhAfrom Lactococcus lactis | This study |
p23S | pZA23trc containing alsS from B. subtilis | This study |
p23SCD | pZA23trc containing alsS from B. subtilis, ilvC and ilvD from Escherichia coli | This study |
pSp | pSAK containing pgl from E. coli | |
pSzp | pSAK containing zwf and pgl from E. coli | This study |
pSzpee | pSzp containing edd and eda from E. coli | This study |