Inducing perylenequinone production from a bambusicolous fungus Shiraia sp. S9 through co-culture with a fruiting body-associated bacterium Pseudomonas fulva SB1

Table 1 Effects of live bacterium P. fulva SB1 (No. 11) on the individual PQ contents in PDA plates and submerged cultures of Shiraia sp. S9

Cultural method	HA	HC	EA	EB	EC
Individual PQ contents in PDA plate (mg/cm²)
Control	2.96 ± 0.14	0.31 ± 0.01	0.49 ± 0.01	0.18 ± 0.01	ND
Co-culture	6.18 ± 0.05**	1.32 ± 0.12**	1.08 ± 0.02*	0.44 ± 0.01*	0.19 ± 0.02**
Intracellular PQs in submerged culture (mg/g DW)
Control	5.33 ± 0.54	1.88 ± 0.13	1.07 ± 0.02	0.26 ± 0.01	0.31 ± 0.01
Co-culture	8.55 ± 1.14**	3.06 ± 0.06*	2.08 ± 0.04*	3.14 ± 0.10**	3.21 ± 0.26**
Extracellular PQs in cultural culture (mg/L)
Control	1.03 ± 0.01	0.71 ± 0.11	0.28 ± 0.03	ND	ND
Co-culture	1.64 ± 0.13*	1.09 ± 0.17*	0.48 ± 0.02*	0.29 ± 0.01**	0.14 ± 0.01*

The procedure of fungal–bacterial confrontation assay and co-culture was the same as specified in Figs. 1 and 2, respectively. Values are mean ± SD from three independent experiments
ND Not detected
* p < 0.05, ** p < 0.01 versus control group

ISSN: 1475-2859