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Table 2 Plasmids and strains used in this study

From: Cascade synthesis of uridine-5′-diphosphate glucuronic acid by coupling multiple whole cells expressing hyperthermophilic enzymes

Strain or plasmid

Characteristics

Source

E. coli strains

 DH5α

 

Thermo Fisher

 BL21 (DE3)

 

Thermo Fisher

 BL21-TmαGP

pET20b-TmαGP

This work

 BL21-StUSP

pET21b-StUSP

This work

 BL21-PiUDH

pET15b-PiUDH

This work

 BL21-TkNOX

pET21b-TkNOX

This work

 BL21-PiUDH-TkNOX

pET15b-PiUDH and pACYCDuet1-TkNOX

This work

Plasmids

 pET20b(+)

Expression vector, ApR

Novagen

 pET21b(+)

Expression vector, ApR

Novagen

 pET15b

Expression vector, ApR

Novagen

 pACYCDuet-1

Expression vector, CmR

Novagen

 pET20b-TmαGP

Expression vector of TmαGP

This work

 pET21b-StUSP

Expression vector of ST0452

This work

 pET15b-PiUDH

Expression vector of PiUDH

This work

 pET20b-TkNOX

Expression vector of TkNOX

This work

 pACYC Duet1-TkNOX

Expression vector of TkNOX

This work