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Table 2 Plasmids and strains used in this study

From: Cascade synthesis of uridine-5′-diphosphate glucuronic acid by coupling multiple whole cells expressing hyperthermophilic enzymes

Strain or plasmid Characteristics Source
E. coli strains
 DH5α   Thermo Fisher
 BL21 (DE3)   Thermo Fisher
 BL21-TmαGP pET20b-TmαGP This work
 BL21-StUSP pET21b-StUSP This work
 BL21-PiUDH pET15b-PiUDH This work
 BL21-TkNOX pET21b-TkNOX This work
 BL21-PiUDH-TkNOX pET15b-PiUDH and pACYCDuet1-TkNOX This work
Plasmids
 pET20b(+) Expression vector, ApR Novagen
 pET21b(+) Expression vector, ApR Novagen
 pET15b Expression vector, ApR Novagen
 pACYCDuet-1 Expression vector, CmR Novagen
 pET20b-TmαGP Expression vector of TmαGP This work
 pET21b-StUSP Expression vector of ST0452 This work
 pET15b-PiUDH Expression vector of PiUDH This work
 pET20b-TkNOX Expression vector of TkNOX This work
 pACYC Duet1-TkNOX Expression vector of TkNOX This work